M8883

4-Methylumbelliferylphosphat

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

• Synonym(e): 4-Methylumbelliferyl-phosphorsäure
• Empirische Formel (Hill-System): C₁₀H₉O₆P
• CAS-Nummer: 3368-04-5
• Molekulargewicht: 256.15
• Beilstein: 1687229
• EG-Nummer: 222-137-7
• MDL-Nummer: MFCD00016969
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24897292
• NACRES: NA.32

Eigenschaften

• Produktbezeichnung: 4-Methylumbelliferylphosphat, phosphatase substrate
• Beschreibung: phosphatase substrate
• Assay: ≥98% (HPLC)
• Form: powder
• Löslichkeit: water: 25 mg/mL, clear to very slightly hazy, colorless
• Fluoreszenz: λ_ex 319 nm; λ_em 384 nm (pH 9.1); λ_ex 360 nm; λ_em 449 nm (Reaction product)
• Lagertemp.: −20°C
• SMILES String: CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
• InChI: 1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
• InChIKey: BCHIXGBGRHLSBE-UHFFFAOYSA-N

Beschreibung

Anwendung

4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay.[1] It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).[2][3]

Biochem./physiol. Wirkung

4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase[4] in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.

Substrate

Fluorogenisches Substrat für Phosphatase.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)