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LY0100

Sigma-Aldrich

Lysozyme Activity Kit

sufficient for 100 tests

Synonym(e):

Lysozyme assay kit

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499,00 €

499,00 €


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1 KIT
499,00 €

About This Item

UNSPSC-Code:
12352202
NACRES:
NA.26

499,00 €


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Verwendung

sufficient for 100 tests

Lagertemp.

2-8°C

Allgemeine Beschreibung

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Anwendung

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme[1], lysozyme purified from chicken egg white[2] and lysozyme from pilocarpine-stimulated saliva.[3]

Biochem./physiol. Wirkung

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.[4]

Lagerung und Haltbarkeit

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • L6876Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg proteinSDB

  • Lysozyme Reaction Buffer

  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cellsSDB

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Silica Nanopollens Enhance Adhesion for Long-Term Bacterial Inhibition.
Song, H., et al.
Journal of the American Chemical Society, 138, 6455-6462 (2016)
Paula Chequer Gouveia Mól et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1044-1045, 17-23 (2017-01-09)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification of lysozyme (LYZ), directly from chicken egg white (EW). The Tris-cryogel presented
Luana E Bomfin et al.
Biochemical pharmacology, 145, 34-45 (2017-09-05)
This study aimed to elucidate the effect of 5-fluorouracil (5-FU) on the histological aspects of the major salivary glands, salivary flow and saliva composition using an established oral mucositis model in hamsters. Oral mucositis was induced by two intraperitoneal administrations
Dan Bi Lee et al.
Scientific reports, 8(1), 2541-2541 (2018-02-09)
Time-resolved serial femtosecond crystallography with X-ray free electron laser (XFEL) holds the potential to view fast reactions occurring at near-physiological temperature. However, production and characterization of homogeneous micron-sized protein crystals at high density remain a bottleneck, due to the lack
Sara Ricci et al.
Frontiers in physiology, 12, 645529-645529 (2021-06-22)
Saliva facilitates feed ingestion, nutrient circulation, and represents an important pH buffer for ruminants, especially for cattle fed high-concentrate diets that promote rumen acidification. This experiment evaluated the short-term effects of nine phytogenic compounds on salivation, saliva physico-chemical composition as

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