K4519

Keratinase

lyophilized powder

• Synonym(e): Keratinase from Bacillus licheniformis, KerA, Keratinolytic protease
• EC-Nummer: 3.4.4.25 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli BL21
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: 300-1000 units/mg protein
• Mol-Gew.: ~39 kDa
• pH-Bereich: 5.5-12.5(optimum activity is seen at pH 12.5)
• pI: 8.73
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Keratinase is a non-specific serine protease that cleaves non-terminal peptide bonds. This enzyme is active between 37°- 70°C. The highest activity is typically seen at 70°C.

Keratinase is also metalloprotease[1] present in bacteria as well as eukaryotes. Keratinase isolated from Bacilus licheniformis culture is a monomeric enzyme.[2]

Anwendung

Keratinase may be used for enzymatic treatment of elementary body (EB), GAG molecules, and cells in the study of the role glycosaminoglycans (GAGs) in the invasion of host cells by Chlamydia pneumoniae strains.[3]

Biochem./physiol. Wirkung

Keratinase is a particular class of extracellular proteolytic inducible enzyme with the capability of degrading insoluble keratin substrates. [4][5] It is important for hydrolyzing hair, feather, and collagen in sewage system during waste water treatment. It is also useful in food industry, animal feed preparation etc.[6] Insoluble feather keratin from poultry industry may be converted by enzymatic hydrolysis to glues, feedstuffs, fertilizers, films or used for the production of rare amino acids serine, cysteine and proline.[4]

Physikalische Eigenschaften

Keratinase is activated by 0.10% SDS, 1.0% CTAB, and EDTA. Keratinase is partially inhibited by Tween^®20, DMSO, isopropanol, methanol, and ethanol.

Einheitendefinition

One unit of enzyme is able to hydrolyze casein resulting in an absorbance value as the Folin-Ciocalteau reagent equivalent to 1 umole (181μg) of tyrosine per minute at pH 7.5 at 37 °C.

Angaben zur Herstellung

The enzyme can be solubilized at 0.5-1.0 mg/mL in either sterile water or phosphate buffer. The best activity is seen with freshly prepared solutions. However, single-use aliquots of Keratinase solutions can be stored at -20° C.

Substrate

Keratin. Keratinases have also been used for the degradation of prion and prion-like proteins.

Sonstige Hinweise

This enzyme contains a C-terminus 6-Histidine tag.

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302,H315,H319,H335
• P-Sätze: P261 - P264 - P271 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3