H9912

Anti-hSNF5/INI1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL-Nummer: MFCD05665222
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 42-45 kDa (doublet)
• Speziesreaktivität: human
• Konzentration: ~1 mg/mL
• Methode(n): indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde/Triton fixed hSNF5/INI1-transfected 293T cell line; microarray: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear extracts
• UniProt-Hinterlegungsnummer: Q12824
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SMARCB1(6598) ; mouse ... Smarcb1(20587)

Beschreibung

Allgemeine Beschreibung

INI1/SNF5, also known as SWItch/sucrose non-fermentable (SWI/SNF) related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, is encoded by the gene mapped to human chromosome 22q11.2. This gene codes for an essential component of chromatin-remodelling SWI/SNF multiprotein complexes.

Immunogen

synthetic peptide corresponding to amino acids 276-288 of human SNF5. The sequence is conserved in mouse.

Anwendung

Anti-hSNF5/INI1 antibody produced in rabbit has been used in microarray and immunofluorescence.

Biochem./physiol. Wirkung

INI1/sucrose non-fermentable 5 (SNF5) plays a vital role in transcriptional activation of inducible genes through chromatin remodelling. hSNF5/INI1 interacts with the protooncogene c-Myc and the SWI complex is necessary for c-Myc mediated transactivation. hSNF5 displays properties of a tumor-suppressor gene, as sporadic rhabdoid tumors show biallelic loss-of-function mutations and germline mutations confer an autosomal-dominant syndrome that predisposes patients to a variety of rhabdoid cancers. Loss of function mutations of the gene is associated with the pathogenesis of aggressive pediatric cancer and epithelioid schwannoma. SNF5 interacts with E1 protein of human papillomavirus (HPV) and thus, enhances the efficiency of HPV DNA replication.

Physikalische Form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)