GRSA

Glutathione Reductase Assay Kit

Sufficient for 100 colorimetric tests

• Synonym(e): Glutathione Reductase Activity Assay Kit
• UNSPSC-Code: 12161503
• NACRES: NA.28

Eigenschaften

• Qualitätsniveau: 200
• Verwendung: sufficient for 100 tests
• Nachweisverfahren: colorimetric
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... GSR(2936)

Beschreibung

Allgemeine Beschreibung

Glutathione reductase (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. 

Anwendung

Glutathione Reductase Assay Kit has been used to measure the activity of glutathione reductase as a part of oxidative stress assessment and also to study the effects of antifouling biocides on it.

Biochem./physiol. Wirkung

Glutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A₃₄₀ caused by the oxidation of NADPH or the increase in A₄₁₂ caused by the reduction of dithiobis(2-nitrobenzoic acid).

This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A₃₄₀ caused by the oxidation of NADPH or the increase in A₄₁₂ caused by the reduction of dithiobis(2-nitrobenzoic acid).

Eignung

Suitable for the measurement of glutathione reductase activity in biological samples

Prinzip

This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).

Hinweis zur Analyse

UV assay: One unit will cause the oxidation of 1.0 μmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 μmole of DTNB to TNB at 25 °C at pH 7.5.

Kit-Komponenten auch einzeln erhältlich

• D8130 5,5′-Dithiobis(2-nitrobenzoic acid) 50 mg
• G4626 Glutathione, oxidized, disodium salt 100 mg
• N6505 β-Nicotinamide adenine dinucleotide 25 mg

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable