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CS0410

Sigma-Aldrich

Glutathion-S-Transferase(GST)-Assay-Kit

sufficient for 500 multiwell tests

Synonym(e):

GST-Enzymaktivitätskit

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1 KIT
716,00 €

716,00 €

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1 KIT
716,00 €

About This Item

EG-Nummer:
202-551-4
UNSPSC-Code:
12161503
NACRES:
NA.84

716,00 €

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Verwendung

sufficient for 500 multiwell tests

Qualitätsniveau

200

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... GSTA4(2941) , GSTM1(2944)

Verwandte Kategorien

Allgemeine Beschreibung

Das Glutathion-S-Transferase(GST)-Assaykit wurde zur Messung der Gesamt-GST-Aktivität entwickelt. Es kann zum Messen der GST-Aktivität in Zell- und Bakterienlysaten, Gewebehomogenaten und Plasma-
und Erythrozytenlysaten eingesetzt werden.

Anwendung

Das Glutathion-S-Transferase-Assaykit nutzt 1-Chlor-2,4-dinitrobenzol (CDNB), das sich für das breiteste Spektrum von GST-Isozymen eignet. Bei der Konjugation der Thiolgruppe von Glutathion an das CDNB-Substrat kommt es zu einer Steigerung der Absorption bei 340 nm.

Biochem./physiol. Wirkung

Glutathion-S-Transferasen (GSTs) sind eine Gruppe von Enzymen, die bei der Entgiftung zahlreicher Xenobiotika in Säugern wichtig sind. Die Enzyme schützen die Zellen vor Giftstoffen, indem sie die Thiolgruppe des Glutathions an elektrophile Xenobiotika konjugieren und die Zellen auf diese Weise vor den mutagenen, karzinogenen und toxischen Wirkungen der Verbindungen schützen. Die GST-Aktivität tritt in Pflanzen, Insekten, Hefen, Bakterien und den meisten Säugergeweben auf, besonders aber in der Leber, der bei der Entgiftung eine wichtige Funktion zufällt.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Dulbecco's Phosphate Buffered Saline 100 mL
  • L-Glutathione Reduced 1 g
  • GST (Control) .1 mL
  • Sample Buffer 5 mL
  • Substrate (CDNB) 1.2 mL

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000423654
0000423654
0000212520
098M4052V
11180225

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure
S Melino et al.
Amino acids, 41(1), 103-112 (2010-03-10)
Natural organosulfur compounds (OSCs) have been shown to have chemopreventive effects and to suppress the proliferation of tumor cells in vitro through the induction of apoptosis. The biochemical mechanisms underlying the antitumorigenic and anti-proliferative effects of garlic-derived OSCs are not
T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
Aleksandra Kołota et al.
International journal of environmental research and public health, 16(13) (2019-07-03)
The World Health Organization (WHO) reported that alcohol consumption is a serious problem in adolescents. The aim of the study was to assess the influence of the time of exposure of various alcoholic beverages on body mass as well as
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Fragen

1-5 von 5 Fragen  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Hilfreich?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Hilfreich?

  3. Is there a protocol available for preparing tissue for assay with the CS0410 Glutathione-S-Transferase (GST) Assay Kit?

    1 Antwort

    1. A specific tissue preparation protocol is not included with this kit. Product C3228, CelLytic MT reagent for mammalian tissue, is suitable. Please see the link below to review the product datasheet and protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Flash freezing the sample in liquid nitrogen and cold homogenization methods are also suitable for use with this kit. There are a number of methods available in published literature. Please see the link below for articles that may be helpful:
      https://scholar.google.com/scholar?q=tissue+lysate+protocol&hl=en&as_sdt=0&as_vis=1&oi=scholart

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  4. Is this suitable for detecting all of GST activity or is there for specific GST family for example GSTA or GSTM activity only?

    1 Antwort

    1. This product has not been evaluated for specificity to each GST isozyme. However, there is no evidence to suggest that this kit does not react to all GST isozymes.

      Hilfreich?

  5. ¿Cuál es el mejor protocolo para su utilización?

    1 Antwort

    1. The product datasheet is listed in the DOCUMENTATION section under 'More Documents'. Please see the links below to access the Product Datasheet:
      https://www.sigmaaldrich.com/product/sigma/cs0410#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

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