GE17-5144-01
HiTrap® Benzamidine Fast Flow
Cytiva 17-5144-01, pack of 1 × 5 mL
About This Item
Empfohlene Produkte
ligand
p-aminobenzamidine
Verpackung
pack of 1 × 5 mL
Hersteller/Markenname
Cytiva 17-5144-01
Lagerbedingungen
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Parameter
42 psi
Bettgröße
16 mm × 25 mm
Bettvolumen
5 mL
Säule ID
16 mm
Matrix
highly cross-linked 4% agarose
Durchschnittlicher Durchmesser
90 μm
cleaning
1-9
working range
2-8
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Anwendung
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Leistungsmerkmale und Vorteile
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Sonstige Hinweise
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
Protokolle
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
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