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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

Synonym(e):

IGM Purification Column

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

Verpackung

pack of 5 × 1 mL

Hersteller/Markenname

Cytiva 17-5110-01

Lagerbedingungen

(20% Ethanol)

Parameter

<4 mL/min flow rate
42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

16 mm

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

2-13

working range

3-11

Lagertemp.

2-8°C

Allgemeine Beschreibung

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

Anwendung

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Leistungsmerkmale und Vorteile

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protokolle

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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