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GE17-1128-01

MAbtrap® Kit

Cytiva 17-1128-01, pkg of 1 kit

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About This Item

UNSPSC-Code:
13111000
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Verpackung

pkg of 1 kit

Hersteller/Markenname

Cytiva 17-1128-01

Lagerbedingungen

(20% Ethanol)

Parameter

<4 mL/min flow rate
42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

7 mm

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

2-9

working range

3-9

Kapazität

>1 mg binding capacity (lgG/column)

Lagertemp.

2-8°C

Allgemeine Beschreibung

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Anwendung

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose High Performance.

Leistungsmerkmale und Vorteile

  • Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
  • Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose High Performance and enough buffer concentrate for 20 preparations with a syringe.
  • MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Piktogramme

Exclamation mark

Signalwort

Warning

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protokolle

This page shows how to prepare samples for purification with affinity chromatography.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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