Fluorescence detection is a preferred method for many quantitative protein microarray assaysin research and diagnostic applications due to its excellent sensitivity and potential for multiplexing. Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wave lengths (such as the 532nm fluorescence channel). These ONCYTE™ SuperNOVA™ microporous nitrocellulose film slides increase protein microarray assay sensitivity and dynamic range using fluorescent end points at multiple wave lengths. Use of SuperNOVA™ slides can lower the limit of detection with fluorescent end points by 3 – 4 orders of magnitude over competing nitrocellulose film slides.
Porous nitrocellulose (PNC) film slides are the major high binding protein microarray substrate technology. Grace Bio-Labs ONCYTE® nitrocellulose film slides offer a significantly higher surface area for protein binding. These slides are compatible with many detection methods. Fluorescent, chemiluminescent, isotopic, and chromogenic detection methods that are commonly employed for Western and Northern blots are compatible with porous nitrocellulose films. ONCYTE® film slides need not require any activation step to immobilize proteins and are printable straight from the box. Pre-wetting ONCYTE® film slides should be avoided as printing onto a wet slide will result in the sample spreading, resulting in larger, more diffuse spots.
Anwendung
Grace Bio-Labs ONCYTE® SuperNOVA™ nitrocellulose film slides might be suitable for:
antigen or antibody capture
cell or tissue lysate binding
glycoprotein and peptide binding assay
Leistungsmerkmale und Vorteile
Packed in boxes with minimal off-gassing
Require no pre-processing prior to array spotting
Ready for printing straight from the box
Require no activation steps to immobilize proteins
Can be analyzed using various laser scanners or colorimetric or fluorescence detectors (CCD) imaging systems
Rechtliche Hinweise
NOVA is a trademark of Grace Bio-Labs, Inc.
Oncyte is a registered trademark of Grace Bio-Labs, Inc.
SuperNOVA is a trademark of Grace Bio-Labs, Inc.
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