G9768

Guanosin 5′-Triphosphat-Agarose

aqueous glycerol suspension

• Synonym(e): 5′-GTP agarose
• MDL-Nummer: MFCD00171655
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Biologische Quelle: bacterial (CORYNEBACTERIUM SP); enzyme from Porcine brain; plant (Sea weed); yeast
• Qualitätsniveau: 200
• Form: aqueous glycerol suspension
• Kennzeichnungsgrad: 1-5 μmol per mL
• Matrix: cross-linked 4% beaded agarose
• Matrixaktivierung: cyanogen bromide
• Matrixanbindung: ribose hydroxyls
• Matrix-Spacer: 11 atoms (adipic acid dihydrazide)
• Lagertemp.: −20°C

Beschreibung

Anwendung

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physikalische Form

Suspension in 50% Glycerin, enthält 0.25 M NaCl und 0.02% Thiomersal

Guanosine 5′-triphosphate–Agarose has been used:

• in chromatographic column for the purification of a translocation stimulating factor[1]
• in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)[2]
• in GTP-Binding assay[3]
• for the depletion of GTP binding proteins in cytosol of rat liver[4]

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3