G2751

Glutamat-Oxalessig-Transaminase Zubereitung aus Schweineherz

Type I, ammonium sulfate suspension, 200-500 units/mg protein

• Synonym(e): L-Aspartate:2-oxoglutarate aminotransferase, Aspartat-Aminotransferase, GOT
• CAS-Nummer: 9000-97-9
• EC-Nummer: 2.6.1.1 (BRENDA, IUBMB)
• EG-Nummer: 232-571-9
• MDL-Nummer: MFCD00131191
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Typ: Type I
• Qualitätsniveau: 200
• Form: ammonium sulfate suspension
• Spezifische Aktivität: 200-500 units/mg protein
• Fremdaktivität: glutamic-pyruvic transaminase ≤0.03%; lactic dehydrogenase ≤0.01%; malic dehydrogenase ≤0.01%
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Anwendung

Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

• in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
• in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
• as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Biochem./physiol. Wirkung

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

Einheitendefinition

Eine Unit verwandelt 1 μmol α-Ketoglutarat zu L-Glutamat pro Minute bei einem pH von 7,5 und 37 °C, in Gegenwart von L-Asparaginsäure. Eine Unit entspricht ~2,000 O.D. (Karmen) Units bei 25 °C.

Physikalische Form

Suspension in 3.0 M (NH₄)₂SO₄, enthält 0.05 M Maleat und 2.5 mM α-Ketoglutarat, pH 6.0

Hinweis zur Analyse

Proteingehalt mittels Biuret-Reaktion bestimmt.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)