F3022
Monoklonales Anti-β-Aktin
clone AC-15, purified from hybridoma cell culture
Synonym(e):
Monoclonal Anti-β-Actin
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Konjugat
FITC conjugate
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
AC-15, monoclonal
Form
buffered aqueous solution
Mol-Gew.
antigen 42 kDa
Speziesreaktivität
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Darf nicht reagieren mit
Dictyostelium discoideum
Methode(n)
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
Isotyp
IgG1
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Allgemeine Beschreibung
Beim Anfärben von ultradünnen Kryo-Gewebeschnitten von Hühner-Kaumagen markiert der Antikörper die Verdichtungszonen (Dense Bodies) und Intermediärfilamente aus Desmin, die aufeinanderfolgende Dense Bodies verbinden, sowie die membranassoziierten Anheftungsplaques (Dense Plaques). Er färbt keine adulten Herz- und Skelettmuskeln an, außer in Spuren, wenn Kontaminationen der Probe mit anderen als Muskelzellen vorhanden sind, oder wenn embryonales Gewebe verwendet wird.
Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.
Immunogen
leicht modifiziertes β-zytoplasmatisches N-terminales Aktin-Peptid, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, konjugiert mit KLH.
Anwendung
Ein monoklonaler Maus-Anti-Aktin-Antikörper wurde als Beladungskontrolle für die Western-Blot-Analyse von Leberproteinlysaten aus Ratten verwendet.
Ein monoklonaler Maus-Anti-Aktin-Antikörper wurde als Beladungskontrolle für die Western-Blot-Analyse von immungefällten Proteinen aus Ratten-Spinalganglien-Co-Kulturen verwendet.
Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- direct immunofluorescent staining
- dual immuno-fluorescent staining
Biochem./physiol. Wirkung
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Sonstige Hinweise
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Analysenzertifikate (COA)
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