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CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Synonym(e):

Thioredoxin Reductase Activity Kit

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Qualitätsniveau

200

Verwendung

 kit sufficient for 100 assays (1 mL)

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)

Verwandte Kategorien

Allgemeine Beschreibung

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Anwendung

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Leistungsmerkmale und Vorteile

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Assay Buffer 5x for Thioredoxin Reductase 30 mL
  • Thioredoxin Reductase 10 μg
  • Thioredoxin Reductase Inhibitor Solution .05 mL
  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
  • NADPH 25 mg
  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Piktogramme

Health hazard
GHS08

Signalwort

Warning

H-Sätze

H361

Gefahreneinstufungen

Repr. 2

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F

Flammpunkt (°C)

87 °C

Analysenzertifikate (COA)

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