PEA-13

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 97040311, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse embryo
• Verpackung: tube of 5 μg 97040311-DNA-5UG; pkg of vial of cells 97040311-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse embryo fibroblast, LRP-deficient, homozygous

Beschreibung der Zelllinie

The cell line PEA-13 is homozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-13 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Pseudomonas exotoxin A (PEA) was used to select murine fibroblasts genetically deficient in LRP. Together with the cell lines PEA-10 (Sigma Catalogue number. 97040310) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP-deficiency. This is of importance since LRP-deficient mouse embryos die early during gestation.

Anwendung

Study of LRP and LRP deficiency.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Sonstige Hinweise

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