ND7/23 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090903, Mouse x Rat Hybridoma nerve, Neuronal

• Synonym(e): ND7/23 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Rat Hybridoma nerve
• Verpackung: tube of 5 μg 92090903-DNA-5UG; pkg of vial of cells 92090903-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Neuronal
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse neuroblastoma x Rat neurone hybrid

Beschreibung der Zelllinie

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

Anwendung

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x10⁴ viable cells/cm² at 37°C and 5% CO₂. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Sonstige Hinweise

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