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C9210
Cyanogenbromid-aktivierte Agarose
lyophilized powder
Synonym(e):
Agarose, cyclic carbonimidate, CNBr-Activated Agarose
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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1 G
113,00 €
5 G
379,00 €
15 G
644,70 €
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1 G
113,00 €
5 G
379,00 €
15 G
644,70 €
About This Item
Empfohlene Produkte
Form
lyophilized powder
Methode(n)
affinity chromatography: suitable
Matrix
cross-linked 4% beaded agarose
Aktive Matrixgruppe
cyanate or related structures
Matrix-Spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
Quellen
1 g swells to 2.5-4.5 mL
Kapazität
≥10 mg/mL binding capacity (BSA)(via amino groups)
Eignung
suitable for chromatography
Lagertemp.
−20°C
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Anwendung
Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.
Physikalische Form
Lyophilisiertes Pulver stabilisiert mit Lactose
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B.
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Hilfreich?
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What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?
1 Antwort-
The control for this product is Product No. CL4B200 (Sepharose CL-4B).
Hilfreich?
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What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?
1 Antwort-
Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare. In addition, C9210 is a cross-linked resin and C9142 is not.
Hilfreich?
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When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?
1 Antwort-
The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.
Hilfreich?
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