C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• Synonym(e): 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS-Nummer: 37289-07-9
• EC-Nummer: 3.5.1.24 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130789
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥100 units/mg protein
• Zusammensetzung: Protein, ≥50% biuret
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Anwendung

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2][3]

Biochem./physiol. Wirkung

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[4]

Einheitendefinition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physikalische Form

Partially purified lyophilized powder containing buffer salts and stabilizer

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)