Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

A3653

Sigma-Aldrich

Aktin aus Rindermuskel

lyophilized powder

Synonym(e):

G-Actin

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

CAS-Nummer:
51005-14-2
MDL-Nummer:
MFCD00130338
UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

bovine muscle

Qualitätsniveau

200

Assay

≥90% (SDS-GE)

Form

lyophilized powder

Mol-Gew.

43 kDa

Zusammensetzung

Protein, ≥80% biuret

Methode(n)

western blot: suitable

UniProt-Hinterlegungsnummer

P62739

Lagertemp.

2-8°C

Angaben zum Gen

bovine ... LOC(528168)

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Allgemeine Beschreibung

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.[1]

Anwendung

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.[2][3]

Biochem./physiol. Wirkung

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction.[1] The protein acts as a building block of microfilaments.[4]

Physikalische Form

Lyophilized powder containing Tris, ATP and CaCl2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
SLBX5919
SLCB8061
SLBQ7282V
SLBM5951V
SLBD7277V

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kunden haben sich ebenfalls angesehen

Slide 1 of 2

1 of 2

Keratin aus Humanepidermis aqueous solution

Sigma-Aldrich

K0253

Keratin aus Humanepidermis

Hämoglobin aus Rinderblut dried erythrocytes

Sigma-Aldrich

H3760

Hämoglobin aus Rinderblut

Contrasting cellular stress responses of Baikalian and Palearctic amphipods upon exposure to humic substances: environmental implications
Protopopova MV, et al.
Environmental Science and Pollution Research International, 21(24), 14124-14137 (2014)
The vitamin D binding protein DBP
Vitamin D, 57-72 (2011)
T V L Berghof et al.
Poultry science, 89(2), 227-235 (2010-01-16)
Natural antibodies (NAb) have been divided in 2 classes: overt and cryptic. Overt NAb can be detected in unfractionated normal sera of nonimmunized mammals and chickens. Cryptic NAb as described in mammals need an in vitro physical or biochemical treatment
Laure Diancourt et al.
Toxins, 11(3) (2019-03-03)
Clostridiumperfringens type E is a less frequently isolated C.perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C.perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and
Rheumatoid synovial fibroblasts constitutively express the fibrinolytic pattern of invasive tumor-like cells
Guiducci S, et al.
Clinical and Experimental Rheumatology, 23(3), 364-372 (2005)
Alle zugehörigen Dokumente anzeigen

Artikel

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Fragen

  1. Can you provide the protocol to polymerize the Actin from Bovine muscle?

    1 Antwort

    1. In the absence of salts, actin exists as a globular monomeric protein (G-actin). In the presence of ATP, a divalent cation (Ca2+ or Mg2+) and high salts (KCl, NaCl or MgCl2), the G-actin polymerizes to form a fibrous or filamentous actin (F-actin) which incorporates the cation and ATP (3,4). Dialysis of the F-actin solution (viscous) against water reverses the polymerization (2). The actin as it is supplied in product A3653 has been extensively dialyzed to be converted into G-Actin.

      This is described in the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/659/a3653pis.pdf

      References
      2. Sigma quality control and production.
      3. Carlier, M.-F., J. Biol. Chem., 266, 1-4 (1991) - citing Kabsch, W. et al., Nature, 347, 37-44 (1990).
      4. Merck Index, 12th Ed., #137 (1996).

      Hilfreich?

Bewertungen

Kein Beurteilungswert

Aktive Filter

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.