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H9773

Sigma-Aldrich

Hygromycin B aus Streptomyces hygroscopicus

lyophilized powder, suitable for plant cell culture, BioReagent

Synonym(e):

Hygromycin

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50 MG
109,00 €
100 MG
187,00 €
250 MG
365,00 €

About This Item

Empirische Formel (Hill-System):
C20H37N3O13
CAS-Nummer:
Molekulargewicht:
527.52
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352207
PubChem Substanz-ID:
NACRES:
NA.72

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Produktbezeichnung

Hygromycin B aus Streptomyces hygroscopicus, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder

Biologische Quelle

Streptomyces hygroscopicus

Qualitätsniveau

Produktlinie

BioReagent

Form

lyophilized powder

Aufgereinigt durch

ion-exchange chromatography

Konzentration

≥60% (HPLC)

Methode(n)

cell culture | plant: suitable

Farbe

faintly brown to brown
white to beige

 

(1) 7.1, (2) 8.8

Löslichkeit

H2O: soluble 50 mg/mL
ethanol: soluble
methanol: soluble

Wirkungsspektrum von Antibiotika

fungi

Anwendung(en)

agriculture

Wirkungsweise

protein synthesis | interferes

Lagertemp.

2-8°C

SMILES String

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChIKey

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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Allgemeine Beschreibung

Chemical structure: aminoglycoside
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.[1]

Anwendung

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression.[2] It is also used in MS medium for the in vitro screening of transgenic plants.[3]

Biochem./physiol. Wirkung

Wirkungsmechanismus: Das Produkt wirkt, indem es die Proteinsynthese durch die Einleitung der Fehllesung der m-RNA-Matrix in Prokaryoten hemmt, mit der Wirkung der Translationshemmung.

Antimikrobielles Spektrum: Hygromycin B wirkt gegen Bakterien, Pilze und höhere eukaryotische Zellen.
Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni.[1] It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans.[4] It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation.[5] The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.[6]

Vorsicht

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

Angaben zur Herstellung

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Piktogramme

Skull and crossbones

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Yajun Xi et al.
Methods in molecular biology (Clifton, N.J.), 581, 53-59 (2009-09-22)
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced
M Dutt et al.
Plant cell reports, 29(11), 1251-1260 (2010-08-17)
A method for the genetic transformation of several citrus cultivars is described, including cultivars observed to be recalcitrant to conventional epicotyl-mediated transformation. Embryogenic cell suspension cultures, established from unfertilized ovules were used as target tissues for Agrobacterium-mediated transformation. Several modifications
Inja Radman et al.
PloS one, 8(10), e76019-e76019 (2013-10-17)
We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with
Andrew Cameron et al.
PloS one, 9(4), e95084-e95084 (2014-04-23)
Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in
Daisuke Iino et al.
Journal of structural biology, 183(1), 76-85 (2013-06-12)
Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) inactivates the aminoglycoside antibiotic hygromycin B (hygB) via phosphorylation. The crystal structure of the binary complex of APH(4)-Ia with hygB was recently reported. To characterize substrate recognition by the enzyme, we determined the crystal

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Protokolle

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