This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.
Wichtige Dokumente
D8418
Dimethylsulfoxid
for molecular biology
Synonym(e):
DMSO für die Molekularbiologie, Methylsulfinylmethan, Methylsulfoxid, DMSO
About This Item
PCR: suitable
transfection: suitable
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Dampfdichte
2.7 (vs air)
Dampfdruck
0.42 mmHg ( 20 °C)
Produktlinie
BioReagent
Assay
≥99.9%
Form
liquid
Selbstzündungstemp.
573 °F
Haltbarkeit
Recommended retest period - 2 years
Expl.-Gr.
42 %, 63 °F
Methode(n)
DNA sequencing: suitable
PCR: suitable
transfection: suitable
Verunreinigungen
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
Farbe
colorless
Brechungsindex
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp (Schmelzpunkt)
16-19 °C (lit.)
Löslichkeit
H2O: miscible (completely)
Dichte
1.10 g/mL (lit.)
Absorption
passes test
Eignung
suitable for molecular biology
Fremdaktivität
DNase and RNase, none detected
Lagertemp.
room temp
SMILES String
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChIKey
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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Allgemeine Beschreibung
- Geeignet für molekulare Anwendungen
- Nuklease-freies DMSO
- DNasefrei, RNasefrei, Proteasefrei, Phosphatasefrei
- DMSO für die Molekularbiologie
Dimethylsulfoxid (DMSO) ist ein hochpolares organisches Reagenz, das über außergewöhnliche Lösungsmitteleigenschaften für organische und anorganische Chemikalien verfügt.
Dieses Produkt ist als molekularbiologisches Reagenz klassifiziert und ist für molekularbiologische Anwendungen geeignet. Es wurde auf das Vorhandensein von Nukleasen und Proteasen hin analysiert.
Anwendung
- Polymerase-Kettenreaktion (PCR)
- Amplifikation von cDNA-Bibliotheken
- DNA-Sequenzierung
- Säulen-Ladepuffer für die Poly(A)+ RNA-Selektion
- Puffer für die Transformation kompetenter E. coli
- Transfektionsprotokolle
Dimethylsulfoxid wird verwendet:
- als Lösungsmittel für Oligonukleotide beim Array-Spotting[1]
- als Lösungsmittel bei der miRNA-Microarray-Analyse und der quantitativen Echtzeit-PCR (qRT-PCR)
- für die Zelllyse während des MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium-Bromid)-Assays
- für die Herstellung der Stammlösung während der zytofluorimetrischen Bewertung des MMP (mitochondriales Membranpotential)
- bei der Lagerung menschlicher und tierischer Zelllinien und Bakteriophage λ als Kryoprotektor.
Leistungsmerkmale und Vorteile
Vorsicht
Als Spritzschutz empfohlene Handschuhe
auch häufig zusammen mit diesem Produkt gekauft
Ähnliches Produkt
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
188.6 °F - closed cup
Flammpunkt (°C)
87 °C - closed cup
Persönliche Schutzausrüstung
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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May I know the concentration (mg/ml) for DMSO (cat no. D8418)?
1 Antwort-
Hilfreich?
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Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?
1 Antwort-
Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:
-Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
-Moderately compatible: polystyrene, ECTFE/ETFE
-Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonatehttps://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf
Hilfreich?
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Is this product sterile?
1 Antwort-
This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
https://www.sigmaaldrich.com/product/sigma/d2650
https://www.sigmaaldrich.com/product/sigma/d2438Hilfreich?
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Hilfreich?
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How does the storage temperature relate to shipping conditions?
1 Antwort-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Hilfreich?
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I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?
1 Antwort-
DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.
Hilfreich?
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What is the molarity of DMSO?
1 Antwort-
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
Hilfreich?
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Is Product D8418, Dimethyl sulfoxide, assayed for DNase, RNase and protease?
1 Antwort-
Yes. It is a molecular biology grade product, and it is tested for DNase, RNase and protease. For a lot to pass none of those enzymes may be detected.
Hilfreich?
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With what materials is Product D8418, Dimethyl sulfoxide, compatible?
1 Antwort-
The compatibility of DMSO with various materials is listed here: Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, PTFE (Teflon) and FEP. Moderately compatible: polystyrene, ECTFE/ETFE. Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate.
Hilfreich?
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What is the DMSO dissolved in?
1 Antwort-
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Hilfreich?
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