Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.
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46960
Fluoreszein Natriumsalz
BioReagent, suitable for fluorescence
Synonym(e):
Acid Yellow 73, D&C; Gelb Nr. 8, NaFl, NaFluo, Natriumfluorescein, Uranin
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25 G
31,30 €
100 G
89,70 €
500 G
321,00 €
About This Item
Empirische Formel (Hill-System):
C20H10Na2O5
CAS-Nummer:
Molekulargewicht:
376.27
Farbindexnummer:
45350
Beilstein:
3833041
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.32
Empfohlene Produkte
Produktlinie
BioReagent
Qualitätsniveau
Form
powder
Methode(n)
titration: suitable
Fluoreszenz
λex 460 nm; λem 515 nm(lit.)
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
Eignung
suitable for fluorescence
SMILES String
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
InChIKey
RGPLGPBQJOQFJS-UHFFFAOYSA-L
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Allgemeine Beschreibung
Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
423.7 °F - Pensky-Martens closed cup
Flammpunkt (°C)
217.6 °C - Pensky-Martens closed cup
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?
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