46955

Fluorescein

for fluorescence, free acid

• Synonym(e): Resorcinphthalein
• Empirische Formel (Hill-System): C₂₀H₁₂O₅
• CAS-Nummer: 2321-07-5
• Molekulargewicht: 332.31
• Farbindexnummer: 45350
• Beilstein: 94324
• EG-Nummer: 219-031-8
• MDL-Nummer: MFCD00005050
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 329757275
• NACRES: NA.32

Eigenschaften

• Qualität: for fluorescence
• Qualitätsniveau: 100
• Methode(n): titration: suitable
• Fluoreszenz: λ_ex 490 nm; λ_em 514 nm in 0.1 M Tris pH 8.0
• Eignung: suitable for fluorescence
• SMILES String: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
• InChIKey: GNBHRKFJIUUOQI-UHFFFAOYSA-N
• Angaben zum Gen: human ... PTPN1(5770) , PTPRF(5792)

Beschreibung

Allgemeine Beschreibung

Fluorescein dye is the most commonly used fluorescent probe. It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation. Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.

Anwendung

Fluorescein has been used:

• in the permeability assay of Caco-2 cells
• to test its suitability to serve as lifetime fluorescence standards
• as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin
• as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)