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35845

Sigma-Aldrich

2′,7′-Dichlorfluoreszeindiacetat

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(e):

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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417,00 €

417,00 €

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5 G
417,00 €

About This Item

Empirische Formel (Hill-System):
C24H14Cl2O7
CAS-Nummer:
2044-85-1
Molekulargewicht:
485.27
Beilstein:
68488
MDL-Nummer:
MFCD00037501
UNSPSC-Code:
12352108
PubChem Substanz-ID:
57649438
NACRES:
NA.32

417,00 €

Versandbereit am09. April 2025Details

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Produktlinie

BioReagent

Assay

≥95% (HPLC)

mp (Schmelzpunkt)

232-234 °C (lit.)

Löslichkeit

DMSO: soluble

Fluoreszenz

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

Eignung

suitable for fluorescence

Lagertemp.

2-8°C

SMILES String

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChIKey

VQVUBYASAICPFU-UHFFFAOYSA-N

Verwandte Kategorien

Allgemeine Beschreibung

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[1]

Anwendung

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.[1]

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.[2]

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
BCCL7891
BCCM2610
BCCL2220
BCCK3188
BCCJ3620

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Die Dokumentenbibliothek aufrufen

Laura Sartori Assunção et al.
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Makoto Ohtake et al.
Scientific reports, 7, 42783-42783 (2017-02-22)
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Marina Parra-Robert et al.
Biomolecules, 9(9) (2019-09-01)
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO2NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeO2NPs is related
Zhen-Wei Zou et al.
Redox biology, 16, 226-236 (2018-03-12)
Thyroid cancer is the most common endocrine carcinoma with increasing incidence worldwide and anaplastic subtypes are frequently associated with cancer related death. Radioresistance of thyroid cancer often leads to therapy failure and cancer-related death. In this study, we found that
Saisai Wei et al.
The international journal of biochemistry & cell biology, 113, 95-102 (2019-06-21)
ZnO nanoparticle (ZnO NP) exposure causes oxidative stress in the respiratory system, leading to pulmonary damage. Activating transcription factor 3 (ATF3) participates in a variety of cellular stress responses. However, the role of ATF3 in ZnO NP genotoxicity and cytotoxicity
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Fragen

1-8 von 8 Fragen  

  1. Can I get the protocol? Thank you

    1 Antwort

    1. A protocol is not available for this compound.

      Please see the link below to review a publication that may be helpful.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/

      Hilfreich?

  2. What is the shelf life of this product, as a powder, and once dissolved?

    1 Antwort

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.

      Hilfreich?

  3. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    1 Antwort

    1. 2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

      Hilfreich?

  4. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    1 Antwort

    1. Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

      Hilfreich?

  5. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  6. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    1 Antwort

    1. Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

      Hilfreich?

  7. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    1 Antwort

    1. Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.

      Hilfreich?

  8. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    1 Antwort

    1. It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

      Hilfreich?

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