The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.
Wichtige Dokumente
P2308
Proteinase K aus Tritirachium album
lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
Synonym(e):
Endopeptidase K
Größe auswählen
67,90 €
Auf LagerDetails
Größe auswählen
About This Item
67,90 €
Auf LagerDetails
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Produktlinie
BioUltra
Form
lyophilized powder
Spezifische Aktivität
≥30 units/mg protein
Mol-Gew.
28.93 kDa
Zusammensetzung
Protein, ≥90% biuret
Verunreinigungen
≤1 ppm DNA
Fremdaktivität
DNAse, Nickase and RNAse, none detected
Versandbedingung
wet ice
Lagertemp.
−20°C
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Verwandte Kategorien
Anwendung
Entfernt Endotoxine, die an kationische Proteine wie Lysozym und Ribonuklease A binden.
Berichten zufolge nützlich für die Isolierung von Leber-, Hefe- und Mungbohnen-Mitochondrien
Bestimmung der Enzymlokalisierung auf Membranen
Behandlung von Paraffinschnitten zur Freilegung von Antigenbindungsstellen zur Antikörpermarkierung.
Verdauung von Proteinen aus Hirngewebeproben für Prionen in der TSE-Forschung.
Biochem./physiol. Wirkung
Einheitendefinition
Als Spritzschutz empfohlene Handschuhe
Vergleichbares Produkt
auch häufig zusammen mit diesem Produkt gekauft
Ähnliches Produkt
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Artikel
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.
Protokolle
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Das Protokoll für das GenElute-Aufreinigungskit für genomische Säugetier-DNA beschreibt die Isolation von reiner DNA mit hoher Molekülmasse aus verschiedenen Säugetierquellen.
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
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Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.
1 Antwort-
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Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?
1 Antwort-
Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.
Hilfreich?
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Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!
1 Antwort-
This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.
Please see the link below to review the product datasheet which includes preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdfHilfreich?
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How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?
1 Antwort-
The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
3) https://elifesciences.org/articles/36187#s4Hilfreich?
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How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.
1 Antwort-
This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
End users would need to optimize final dilutionHilfreich?
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the recommended pH range for Product P2308, Proteinase K?
1 Antwort-
Proteinase K is normally used in the pH range of 7.5-8.0.
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How should I store solutions of Product P2308, Proteinase K?
1 Antwort-
Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.
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What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?
1 Antwort-
Amounts for typical usage is 50-200 μg/mL.
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How should I prepare solutions of Product P2308, Proteinase K?
1 Antwort-
Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.
Hilfreich?
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