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P2308

Sigma-Aldrich

Proteinase K aus Tritirachium album

Proteinase K from Tritirachium album

lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

Synonym(e):

Endopeptidase K

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

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5 MG
67,90 €
10 MG
73,20 €
25 MG
102,00 €
100 MG
311,00 €
500 MG
1.010,00 €
1 G
1.990,00 €

67,90 €


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Hersteller von Diagnostika sollten Produkte einer anderen Qualität verwenden: SRE0005

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5 MG
67,90 €
10 MG
73,20 €
25 MG
102,00 €
100 MG
311,00 €
500 MG
1.010,00 €
1 G
1.990,00 €

About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

67,90 €


Auf LagerDetails

Hersteller von Diagnostika sollten Produkte einer anderen Qualität verwenden: SRE0005

Bulk-Bestellung anfordern

Qualität

for molecular biology

Qualitätsniveau

Produktlinie

BioUltra

Form

lyophilized powder

Spezifische Aktivität

≥30 units/mg protein

Mol-Gew.

28.93 kDa

Zusammensetzung

Protein, ≥90% biuret

Verunreinigungen

≤1 ppm DNA

Fremdaktivität

DNAse, Nickase and RNAse, none detected

Versandbedingung

wet ice

Lagertemp.

−20°C

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Anwendung

Nützlich zur proteolytischen Inaktivierung von Nukleasen bei der Isolierung von DNA und RNA.
Entfernt Endotoxine, die an kationische Proteine wie Lysozym und Ribonuklease A binden.
Berichten zufolge nützlich für die Isolierung von Leber-, Hefe- und Mungbohnen-Mitochondrien
Bestimmung der Enzymlokalisierung auf Membranen
Behandlung von Paraffinschnitten zur Freilegung von Antigenbindungsstellen zur Antikörpermarkierung.
Verdauung von Proteinen aus Hirngewebeproben für Prionen in der TSE-Forschung.
Proteolytische Deaktivierung von Nucleasen während der Isolation von DNA und RNA

Biochem./physiol. Wirkung

Proteinase K ist eine stabile und hochreaktive Serinprotease. Ergebnisse von Kristall- und Molekularstrukturstudien lassen darauf schließen, dass das Enzym zur Subtilisin-Familie mit einer katalytischen Triade (Asp39-His69-Ser224) im aktiven Zentrum gehört. Es ist in einer Reihe von Umgebungen stabil: pH, Puffersalze, Detergenzien (SDS) und Temperatur. In Gegenwart von 0,1–0,5 % SDS bleibt Proteinase K aktiv und verdaut zahlreiche Proteine und Nukleasen in DNA-Präparationen, ohne die Integrität der isolierten DNA zu beeinträchtigen.

Einheitendefinition

Eine Einheit hydrolisiert harnstoffdenaturiertes Hämoglobin und erzeugt eine Färbung, die 1,0 μmol Tyrosin je min. bei einem pH-Wert von 7,5 und 37 °C (Farbe von Folin-Ciocalteu-Reagens) entspricht.

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Beschreibung
Preisangaben

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Ähnliches Produkt

Piktogramme

Health hazardExclamation mark

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Mamoru Oshiki et al.
Frontiers in microbiology, 9, 830-830 (2018-05-15)
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed
Mark F Sabbagh et al.
eLife, 7 (2018-09-07)
Vascular endothelial cell (EC) function depends on appropriate organ-specific molecular and cellular specializations. To explore genomic mechanisms that control this specialization, we have analyzed and compared the transcriptome, accessible chromatin, and DNA methylome landscapes from mouse brain, liver, lung, and
Margarita Zayas et al.
PLoS pathogens, 12(1), e1005376-e1005376 (2016-01-05)
Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII
Kumiko Muta et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 31(10), 1615-1623 (2016-05-18)
Although histone acetylation, an epigenetic modification, has been reported to be related to the progression of various diseases, its involvement in nephrosclerosis is unclear. Dahl salt-sensitive rats were used as a model of nephrosclerosis in this study. The rats were
Bahram Peivastegan et al.
BMC plant biology, 19(1), 262-262 (2019-06-19)
Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed

Artikel

Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

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Protokolle

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Das Protokoll für das GenElute-Aufreinigungskit für genomische Säugetier-DNA beschreibt die Isolation von reiner DNA mit hoher Molekülmasse aus verschiedenen Säugetierquellen.

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

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Fragen

1-10 von 10 Fragen  
  1. Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.

    1 Antwort
    1. The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.

      Hilfreich?

  2. Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?

    1 Antwort
    1. Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.

      Hilfreich?

  3. Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!

    1 Antwort
    1. This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.

      Please see the link below to review the product datasheet which includes preparation instructions:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdf

      Hilfreich?

  4. How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?

    1 Antwort
    1. The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
      1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
      2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
      3) https://elifesciences.org/articles/36187#s4

      Hilfreich?

  5. How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.

    1 Antwort
    1. This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
      For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
      End users would need to optimize final dilution

      Hilfreich?

  6. What is the Department of Transportation shipping information for this product?

    1 Antwort
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  7. What is the recommended pH range for Product P2308, Proteinase K?

    1 Antwort
    1. Proteinase K is normally used in the pH range of 7.5-8.0.

      Hilfreich?

  8. How should I store solutions of Product P2308, Proteinase K?

    1 Antwort
    1. Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM  calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.

      Hilfreich?

  9. What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?

    1 Antwort
    1. Amounts for typical usage is 50-200 μg/mL.

      Hilfreich?

  10. How should I prepare solutions of Product P2308, Proteinase K?

    1 Antwort
    1. Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.

      Hilfreich?

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