12158167001

Anti-HA-Biotin, High Affinity (3F10)

from rat IgG₁

• Synonym(e): antibody
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Konjugat: biotin conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 3F10, monoclonal
• Form: lyophilized (stabilized)
• Verpackung: pkg of 50 μg
• Hersteller/Markenname: Roche
• Isotyp: IgG1
• Epitopsequenz: YPYDVPDYA
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.

Spezifität

Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Anwendung

Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:

• Dot blots
• ELISA (enzyme-linked immunosorbent assay)
• Western blots

Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.[1][2][3]

Qualität

Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.

Angaben zur Herstellung

Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:

• Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
• Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
• Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
• Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
• Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.

Working concentration: Working concentration of conjugate depends on application and substrate

The following concentrations should be taken as a guideline:

• Dot blot: 100 ng/ml
• ELISA: 100 ng/ml
• Western blot: 100 ng/ml

Rekonstituierung

Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317,H412
• P-Sätze: P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501
• Gefahreneinstufungen: Aquatic Chronic 3 - Skin Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash