Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

SCM075

Sigma-Aldrich

ROCK-Inhibitor (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Synonym(e):

Y-27632

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Qualitätsniveau

100

Form

liquid

Mol-Gew.

320.26 g/mol

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Verwandte Kategorien

Allgemeine Beschreibung

Der ROCK-Inhibitor (Y-27632) ist ein selektiver, ATP-kompetitiver Inhibitor der Rho-assoziierten Proteinkinase (ROCK), einschließlich p160Rock (Ki=140nM) und Rock-II. Agiert auch als potenter Inhibitor der agonist-induzierten Ca2+-Sensibilisierung der Myosinphosphorylierung und Kontraktion der glatten Muskulatur. Eine einstündige Behandlung mit 10uM Y-27632 blockiert die Apoptose dissoziierter kultivierter menschlicher embryonaler Stammzellen (hESCs) und erhöht das Überleben und die Kloneffizienz von hESCs, ohne ihre Pluripotenz zu beeinträchtigen1. Vor kurzem ging aus einigen Studien hervor, dass die Anwendung von Y-27632 und Accutase (Kat. Nr. SCR005), einer formulierten Mischung von Verdauungsenzymen, die Überlebensrate einzelner hESCs nach dem Auftauen erhöht, wenn konventionelle Methoden des langsamen Einfrierens und schnellen Auftauens angewendet werden.

Lagerung und Haltbarkeit

Vor der Rekonstitution: Lichtgeschützt bei -20 ºC 2 Jahre lang haltbar. Nach der Rekonstitution: Lichtgeschützt bei -20 ºC 1 Jahr lang

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Malgorzata Bienkowska-Haba et al.
Journal of virology, 94(3) (2019-11-22)
It is established that the host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immortalized keratinocytes are altered in the presence of HPV genomes. However, the establishment of HPV-harboring cell lines requires selection and immortalization, which makes it
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Ambra Gianotti et al.
Frontiers in pharmacology, 9, 1176-1176 (2018-11-13)
Cystic fibrosis (CF) is a chronic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, which encodes for a channel expressed at the apical surface of epithelial tissues. Defective chloride and bicarbonate secretion, arising from CFTR mutations
Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
Fibrosis is characterized by hardening, overgrowth, and development of scars in various tissues as a result of faulty reparative processes, diseases, or chronic inflammation. During the fibrotic process in the corneal stroma of the eye, the resident cells called keratocytes
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
Alle zugehörigen Dokumente anzeigen

Artikel

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

Alle anzeigen

Protokolle

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Forskolin-Induced Swelling Assays using Millicell® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Alle anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.