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MABN2304

Sigma-Aldrich

Anti-Legumain/Asparaginyl endopeptidase antibody Clone 6E3

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.74

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

6E3, monoclonal

Mol-Gew.

calculated mol wt 49.41 kDa
observed mol wt ~55 kDa

Aufgereinigt durch

using protein G

Speziesreaktivität

human, mouse

Verpackung

antibody small pack of 100 μg

Methode(n)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

Epitopsequenz

Unknown

Protein-ID-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... LGMN(5641)

Allgemeine Beschreibung

Legumain (UniProt: Q99538; also known as EC: 3.4.22.34, Asparaginyl endopeptidase, AEP, Protease cysteine 1, Delta Secretase) is encoded by the LGMN (also known as PRSC1) gene (Gene ID: 5641) in human. AEP is a lysosomal endopeptidase that displays strict specificity for the hydrolysis of asparaginyl bonds and can simultaneously cleave both amyloid precursor protein (APP) and tau. It cleaves aspartyl bonds slowly, especially under acidic conditions. AEP is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 324-433) that are cleaved off in the mature form. It exists a homodimer before removal of the propeptide and after autocatalytic processing exists as a monomer. In its zymogen form, the uncleaved propeptide blocks access to the active site. AEP is phosphorylated on serine 226 by serine-arginine protein kinase 2 (SRPK2), which accelerates its autocatalytic cleavage and enhances its enzymatic activity. The active form translocates to cytoplasm. In Alzheimer s disease brains AEP is reported to be highly phosphorylated. Depletion of AEP is shown to reduce amyloid peptide production and senile plaque formation in 5XFAD mouse brain that leads to restoration of synaptic activity and cognitive function. Three isoforms of AEP have been described that are produced by alternative splicing. (Ref.: Wang Z-H., et al. (2017). Mol. Cell 67(5); 812-825; Zhang, Z et al (2015). Nat. Commun. 6; 9762).

Spezifität

Clone 6E3 is a mouse monoclonal antibody that specifically detects Legumain (AEP).

Immunogen

His-tagged full-length recombinant human legumain.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Legumain/AEP in SH-SY5Y cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Legumain/AEP in mouse brain tissue lysate.

ELISA Analysis: A representative lot detected Legumain/AEP in ELISA applications (Mattock, K.L., et al. (2010). Atherosclerosis.208(1):83-9; Kang, S.S., et al. (2018). EMBO J. 37(12):e98878).

Immunoprecipitation Analysis: A representative lot immunoprecipitated legumain/AEP in Immunoprecipitation applications (Zhang, Z., et al. (2015). Nat Commun. 6:8762).

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Legumain/AEP in human placenta tissue sections.

Immunocytochemistry Analysis: A representative lot detected Legumain/AEP in Immunocytochemistry applications (Li, D.N., et al. (2003). J. Biol. Chem. 278(40):38980-90; Wang, Z-H., et al. (2019). Cell Rep. 28(3):655-669).

Western Blotting Analysis: A representative lot detected Legumain/AEP in Western Blotting applications (Probst-Kepper, M., et al. (2009). J Cell Mol Med.;13(9B):3343-57; Zhang, Z., et al. (2015). Nat Commun.;6:8762; Wang, Z-H., et al. (2017). Mol Cell.;67(5):812-825; Zhang, Z., et al. (2017). Nat Struct Mol Biol.;24(8):632-642; Kang, S.S., et al. (2018). EMBO J.;37(12):e98878; Wang, Z-H., et al. (2018). Nat Commun.;9(1):1784; Wang, Z-H., et al. (2018). JCI Insight.;3(16):e99007; Wang, Z-H., et al. (2019). Cell Rep.;28(3):655-669).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LGMN/AEP, clone 6E3, Cat. No. MABN2304, is a mouse monoclonal antibody that detects Legumain and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Physikalische Form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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