71077

X-Gal-Lösung – Novagen

Convenience for blue/white screening

• Synonym(e): X-Gal solution
• UNSPSC-Code: 12352200
• NACRES: NA.54

Eigenschaften

• Qualitätsniveau: 100
• Form: solution
• Hersteller/Markenname: Novagen^®
• Lagerbedingungen: OK to freeze
• Methode(n): tissue culture: suitable
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing.[1] X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

Anwendung

X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the Apc^Δ242 mutation in the tissue sample.[2]

Warnhinweis

Toxizität: Reizstoff (B)

Rechtliche Hinweise

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): 188.6 °F
• Flammpunkt (°C): 87 °C