70239-M

His•Bind^®-Aufreinigungskit

• Synonym(e): IMAC, Protein purification
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Hersteller/Markenname: Novagen^®
• Qualitätsniveau: 400
• Lagerbedingungen: OK to freeze
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

HIS-BIND^® purification kit includes pre-tested buffers specifically designed for IDA-based HIS-BIND Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the HIS TAG^®/HIS-BIND^® technology, that leverages the affinity between neighboring histidine in the HIS TAG sequence and immobilized metal ions (such as Ni²⁺ or Co²⁺).

Anwendung

HIS-BIND^® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography[1], the purification of virus binding protein specific for Poliovirus Type 1[2], the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine[3], the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy[4]

Leistungsmerkmale und Vorteile

• Includes HIS-BIND^® resin, HIS-BIND^® buffer kit and chromatography columns.
• Solutions are included for Ni²⁺ charging, binding, washing, and elution.
• Columns are pre-packed with 1.25 ml bed volume of Ni²⁺-charged HIS-BIND resin.

Komponenten

•10 mlHis•Bind-Harz

•1His•Bind-Puffer-Kit

•Pack./4Chromatographiesäulen

Warnhinweis

Toxizität: Mehrere Toxizitätswerte, siehe MSDS (O)

Sonstige Hinweise

Aufgrund der Beschaffenheit der in dieser Lieferung enthaltenen Gefahrgüter können zusätzliche Versandkosten bei Ihrer Bestellung anfallen. Bestimmte Größen sind ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen. Um weitere Informationen über diese Versandzuschläge zu erhalten, kontaktieren Sie bitte Ihren lokalen Vertrieb.

Rechtliche Hinweise

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS02,GHS08,GHS05,GHS09
• Signalwort: Danger
• H-Sätze: H226,H314,H317,H334,H341,H350i,H360D,H372,H373,H411
• P-Sätze: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
• Zielorgane: respiratory tract irritation
• Lagerklassenschlüssel: 3 - Flammable liquids
• Flammpunkt (°F): 86.0 °F
• Flammpunkt (°C): 30 °C