556881
Ribonuclease Inhibitor, Human, Recombinant, E. coli
The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
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About This Item
Empfohlene Produkte
Qualitätsniveau
Form
liquid
Spezifische Aktivität
≥20,000 units/mL
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Fremdaktivität
Endonuclease and RNase, none detected
Versandbedingung
wet ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Biochem./physiol. Wirkung
Cell permeable: no
Primary Target
inactivates RNase
inactivates RNase
Product does not compete with ATP.
Reversible: no
Warnhinweis
Toxicity: Standard Handling (A)
Einheitendefinition
One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.
Physikalische Form
In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.
Rekonstituierung
Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.
Sonstige Hinweise
Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Lee, F.S., et al. 1989. Biochemistry 28, 225.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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