803618

DTME (dithio-bis-maleimidoethane)

• Synonym(e): 1,1′-(dithiodi-2,1-ethanediyl)bis-1H-Pyrrole-2,5-dione, 1-[2-[[2-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]dithio]ethyl]-1H-pyrrole-2,5-dione, DTME
• Empirische Formel (Hill-System): C₁₂H₁₂N₂S₂O₄
• CAS-Nummer: 71865-37-7
• Molekulargewicht: 312.36
• MDL-Nummer: MFCD01863385
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 329768821
• NACRES: NA.22

Eigenschaften

• Assay: ≥90%
• Form: powder
• Mol-Gew.: 312.37
• Eignung der Reaktion: reagent type: cross-linking reagent
• Lagerbedingungen: desiccated
• Löslichkeit: DMSO or DMF: soluble
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• SMILES String: O=C(C=CC1=O)N1CCSSCCN2C(C=CC2=O)=O
• InChI: 1S/C12H12N2O4S2/c15-9-1-2-10(16)13(9)5-7-19-20-8-6-14-11(17)3-4-12(14)18/h1-4H,5-8H2
• InChIKey: SGVWDRVQIYUSRA-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

DTME is a homobifunctional, maleimide crosslinker for conjugation between sulfhydryl groups (-SH). Such bismaleimide crosslinkers are commonly used to explore and characterize protein structure (i.e., oligomerization) or protein interactions. DTME is similar in length to BMH but differs in containing a disulfide bond in its spacer arm, allowing crosslinks with DTME to be cleaved with reducing agent such as dithiothreitol (DTT).

Leistungsmerkmale und Vorteile

• Reactive groups: maleimide (both ends)
• Reactive towards: sulfhydryl groups
• Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
• Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
• Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent

Vorsicht

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302 + H312 + H332,H315,H319,H335
• P-Sätze: P261 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable