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M3913

SigmaMarker™

low range, mol wt 6,500-66,000 Da

Synonym(s):

protein markers, protein standards

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Pack SizeSKUAvailabilityPrice
1 vial
Please contact Customer Service for Availability
CZK 1,860.00
10 vials
Please contact Customer Service for Availability
CZK 8,760.00
CZK 7,446.00

About This Item

UNSPSC Code:
41105337
NACRES:
NA.25
MDL number:
MFCD00165844
Form:
lyophilized powder
Storage temp.:
2-8°C

CZK 1,860.00

Please contact Customer Service for Availability
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Quality Segment

200

form

lyophilized powder

usage

sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm),  vial sufficient for 50-100 PhastGels

mol wt

6,500-66,000 Da

storage temp.

2-8°C

Application

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

Preparation Note

Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.

Other Notes

Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Will yield eight protein bands with Coomassie Blue staining.

Legal Information

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

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Show Differences

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This Item
S8445C6210C1992
Supelco

Supelco

M3913

SigmaMarker™

SigmaMarker™ wide range, mol wt 6,500-200,000 Da

Sigma-Aldrich

S8445

SigmaMarker™

Color Marker Ultra-low Range (M.W. 1,060-26,600)

Supelco

C6210

Color Marker Ultra-low Range (M.W. 1,060-26,600)

ColorBurst™ Electrophoresis Marker mol wt 8,000-220,000 Da

Sigma-Aldrich

C1992

ColorBurst™ Electrophoresis Marker

Quality Level

200

Quality Level

200

Quality Level

100, 200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

liquid

form

solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

6,500-66,000 Da

mol wt

6,500-200,000 Da

mol wt

-

mol wt

8,000-220,000 Da

usage

sufficient for 10-20 large gels (16 × 18 cm),  vial sufficient for 50-100 PhastGels

usage

sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm),  vial sufficient for 50-100 PhastGels

usage

sufficient for 20 applications (on 10 cm × 10 cm mini gels)

usage

-

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pictograms

CorrosionHealth hazard
GHS05,GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000512195
0000512197
0000512195
0000512197
0000309162

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Global Trade Item Number

SKUGTIN
M3913-1VL04061835490141
M3913-10VL04061838256065

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