Reconstitute each vial with 100 uL of deionized water. Vortex for a few seconds to ensure the material is completely dissolved. After reconstitution, immediately aliquot and store the unused portion below –20 °C. Repeated freezing and thawing of reconstituted SigmaMarkers is not recommended.
S8445
SigmaMarker™
wide range, mol wt 6,500-200,000 Da
Synonym(s):
protein markers, protein standards
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | CZK 2,550.00 |
10 vials | Please contact Customer Service for Availability | CZK 11,900.00 |
About This Item
Form:
lyophilized powder
Storage temp.:
2-8°C
CZK 2,550.00
Please contact Customer Service for Availability
Quality Segment
form
lyophilized powder
usage
sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm), vial sufficient for 50-100 PhastGels
mol wt
6,500-200,000 Da
storage temp.
2-8°C
Application
SigmaMarker has been used as a protein marker in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Preparation Note
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
Other Notes
Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)
Will yield 12 protein bands with Coomassie Blue staining.
Legal Information
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100, 200 |
| form lyophilized powder | form lyophilized powder | form solution | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 6,500-200,000 Da | mol wt 6,500-66,000 Da | mol wt 8,000-220,000 Da | mol wt - |
| usage sufficient for 10-20 large gels (16 × 18 cm), vial sufficient for 50-100 PhastGels | usage sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm), vial sufficient for 50-100 PhastGels | usage - | usage sufficient for 20 applications (on 10 cm × 10 cm mini gels) |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Quería saber con que tengo que rehidratar el marcador, si puedo rehidratar solo para una electroforesis (cuanto utilizo y en cuanto lo rehidrato?) La conservación del resto que quedara liofilizado, en nevera o congelado?
1 answer-
Helpful?
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Can the marker be used when the staining method used is Silver staining? Is the marker compatible with Gel and buffers from other makers too. We are considering purchasing the marker for using also as control in Proteomics analysis. Regards,
1 answer-
This product is suitable for silver staining and compatible with gels and buffers from other suppliers. Please see the link below to review the product technical bulletin, including the recommended protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/421/562/s8445bul.pdfHelpful?
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