This product is a beta-lactamase blend containing different enzymes to cover a broad spectrum of beta-lactam antibiotic substrates. No specific molecular weight range is provided. However, information in BRENDA indicates that most of these recombinant enzymes fall within the range of approximately 30 kDa.
L6170
β-Lactamase
recombinant, expressed in E. coli
Synonym(s):
Carbapenemase, Cephalosprinase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Please contact Customer Service for Availability | CZK 16,500.00 |
CZK 16,500.00
Please contact Customer Service for Availability
recombinant
expressed in E. coli
Quality Segment
form
powder
specific activity
≥20 U/mg (with cephalosporin C), ≥400 U/mg (with benzylpenicilin)
storage temp.
2-8°C
General description
β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria.[1] There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis. Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs). As close to 900 types of β-Lactamases are produced by microbes.
Application
β-Lactamase has been used in coumarin-cephalosporin-fluorescein-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression[2]. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring[2]. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Physical form
Lyophilized powder containing sodium chloride and potassium phosphate.
Other Notes
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥20 U/mg (with cephalosporin C), ≥400 U/mg (with benzylpenicilin) | specific activity 0.2-0.6 units/mg protein (using benzylpenicillin) | specific activity - | specific activity ≥1500.00 units/mg protein (using benzylpenicillin) |
| form powder | form lyophilized powder | form lyophilized | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| L6170-1VL | 04061833958667 |
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What is the molecular weight and class of this beta-lactamase?
1 answer-
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Hi. I have a question about product L6170. Is the product in lyophilized form and if yes, how many grams of powder each vial contains?
1 answer-
Yes, this is a lyophilized product. However, the preparation includes sodium chloride and potassium phosphate salts. The amount of material is each vial varies from lot to lot and may be calculated based on analytical data reported in the Certificate of Analysis. For example, a lot that reports 1289 units per vial, 806 units per mg protein, and 0.87% protein, will contain 184 milligrams per vial based on the following calculation:
Units per vial / (units per mg protein x % protein) = milligrams per vial
Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l6170#product-documentationHelpful?
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What is the volume needed to resuspend the lyophilized enzyme?
1 answer-
To reconstitute, it is recommended to dissolve the contents of one vial in 10 mL of deionized, purified water. After reconstitution, filter-sterilize through a 0.2 µm low-protein binding membrane.
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