This product is not specifically tested for cloxacillin neutralization. However, based on the known activity of this product, this material is expected to inactivate cloxacillin by hydrolyzing the beta-lactam ring. Cloxacillin is a beta-lactam antibiotic.
P4524
β-Lactamase from Enterobacter cloacae
Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)
Synonym(s):
β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
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| Size/SKU | Availability | Price |
|---|---|---|
100 units | Please contact Customer Service for Availability | 9 440,00 CZK |
250 units | Please contact Customer Service for Availability | 23 400,00 CZK |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
0.2-0.6 units/mg protein (using benzylpenicillin)
Biological source:
bacterial (Enterobacter cloacae)
9 440,00 CZK
Please contact Customer Service for Availability
biological source
bacterial (Enterobacter cloacae)
Quality Segment
type
Type IV
form
lyophilized powder
specific activity
0.2-0.6 units/mg protein (using benzylpenicillin)
mol wt
29 kDa
composition
Protein, ~60%
technique(s)
enzyme immunoassay: suitable
suitability
suitable for enzyme test
application(s)
pharmaceutical
storage temp.
2-8°C
General description
β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.
Application
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.
β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
Physical form
Lyophilized powder containing sodium phosphate buffer salts
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
1 of 1
This Item | |||
|---|---|---|---|
| description Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin) | description lyophilized, powder, white, ~13 U/mg | description lyophilized powder, ≥1500.00 units/mg protein (using benzylpenicillin) | description lyophilized powder, recombinant, expressed in E. coli |
| specific activity 0.2-0.6 units/mg protein (using benzylpenicillin) | specific activity ~13 U/mg | specific activity ≥1500.00 units/mg protein (using benzylpenicillin) | specific activity - |
| biological source bacterial (Enterobacter cloacae) | biological source Bacillus sp. (Bacillus cereus) | biological source Bacillus sp. (Bacillus cereus) | biological source - |
| technique(s) enzyme immunoassay: suitable | technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) - |
| application(s) pharmaceutical | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| form lyophilized powder | form powder | form lyophilized powder | form lyophilized powder |
| suitability suitable for enzyme test | suitability suitable for component for culture media | suitability - | suitability - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Is it capable for neutralizing cloxacillin 500mg?
1 answer-
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A cuántas UI corresponde la concentración de 0.2-0.6 UNITS del producto?
1 answer-
The International Unit (I.U.), using benzylpenicillin, is approximately equal to 600 Levy or 75 Pollock units. Also, 1 I.U. is approximately 70,000 kinetic Kersey units. Please also note that 1 I.U. = 1 Sigma unit using benzylpenicillin as a substrate. We have no information regarding the classification of the penicillinase enzymes P0389, P6018, P4399, P4524, and P3553 into groups 2b and group 1 of Bush′s classification system. These enzymes are classified according to Enzyme Nomenclature Biology published by the International Union of Biochemistry and Molecular Biology (IUBMB). The penicillinases and cephalosporinases belong to the EC 3.5.2.6 beta-lactamase, defined as a group of enzymes of varying specificity hydrolyzing β-lactams.
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