C6357

Caspase 4 human

recombinant, expressed in E. coli, 5,000 units/mg protein

• Synonym(s): ICE(rel)II, Ich-2, TX
• UNSPSC Code: 12352200
• NACRES: NA.32
• EC Number: 3.4.22.57 (BRENDA, IUBMB)
• MDL number: MFCD03456356
• Specific activity: 5,000 units/mg protein
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• form: solution
• specific activity: 5,000 units/mg protein
• mol wt: 30 kDa
• solubility: PBS: soluble
• UniProt accession no.: P49662
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CASP4(837)

Description

General description

The caspase 4 (CASP4) gene is mapped to human chromosome 11q21.[1] Caspase 4 is a cysteine-aspartic protease,[2] localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).[2]

Biochem/physiol Actions

Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

Caspase 4 is an inflammatory caspase associated with innate immune responses.[2] It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).[2]

Physical form

Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride

Other Notes

One unit will hydrolyze 1 nmol of the caspase substrate WEHD-pNA to WEHD and p-nitroaniline per hour at pH 7.2 at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable