92544
Abberior® FLIP 565, maleimide
for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352111
Pricing and availability is not currently available.
Quality Level
form
solid
concentration
≥50.0% (degree of coupling)
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 565 nm; λem 580 nm±5 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)
Application
Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.[1][2]
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
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This Item | |||
|---|---|---|---|
| concentration ≥50.0% (degree of coupling) | concentration ≥50% (degree of coupling) | concentration - | concentration ≥50.0% (degree of coupling) |
| fluorescence λex 565 nm; λem 580 nm±5 nm in PBS, pH 7.4 | fluorescence λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4 | fluorescence λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4 | fluorescence λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4 |
| solubility DMF: 0.25 mg/mL, clear | solubility DMF: 0.25 mg/mL, clear | solubility DMF: 1 mg/mL, clear | solubility DMF: 0.25 mg/mL, clear |
| form solid | form - | form - | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Related Content
Remi Galland et al.
Nature methods, 12(7), 641-644 (2015-05-12)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 92544-1MG | 04061823360227 |