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38361

Abberior® STAR 440SXP, maleimide

for long Stokes STED and 2-color STED application

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1 mg
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CZK 20,700.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352111

CZK 20,700.00


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Quality Segment

assay

≥80.0% (degree of coupling)

mol wt

Mw 622.6 g/mol

solubility

DMF: 1 mg/mL, clear

fluorescence

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

Application

Abberior® STAR 440SXP has been conjugated with secondary anti-mouse antibody for dual colour STED (stimulated emission depletion) microscopy.[1] Again, it has been labelled with secondary antibody for STED microscopy in primary hippocampal cells prepared from E18 Sprague Dawley embryos.[2]
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH

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This Item
682210300430558
fluorescence

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

fluorescence

λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4

fluorescence

λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4

fluorescence

λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥80.0% (degree of coupling)

assay

≥80.0% (degree of coupling)

assay

-

assay

-

mol wt

Mw 622.6 g/mol

mol wt

Mw 597.5 g/mol

mol wt

Mw 861.7 g/mol

mol wt

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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I-Hsuan Wang et al.
Cell host & microbe, 14(4), 468-480 (2013-10-22)
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome
Linda Westin et al.
BMC neuroscience, 15, 45-45 (2014-03-29)
Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution. Here we have analyzed the spatial relationship between Norbin
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We



Global Trade Item Number

SKUGTIN
38361-1MG04061823366212

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