PC474

Anti-PCNA (247-261) (Ab-5) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-Proliferating Cell Nuclear Antigen
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: human
• Application: —
• Citations: 1

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoprecipitation (1:250-1:1000))
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PCNA(5111)

Description

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.

Recognizes the ~37 kDa PCNA protein in MCF-7 cells.

This Anti-PCNA (247-261) (Ab-5) Rabbit pAb is validated for use in Immunoprecipitation for the detection of PCNA (247-261) (Ab-5).

Immunogen

Human

a synthetic peptide corresponding to amino acids 247-261 of human PCNA

Application

Immunoprecipitation (1:250-1:1000)

Physical form

Undiluted serum.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
MCF7 cells

Other Notes

Miyachi, K., et al. 1990. J. Immunol.121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86, 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.

Will immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1