MABE288
Anti-PCNA Antibody, clone PC10
clone PC10, from mouse
Synonym(s):
Proliferating cell nuclear antigen, PCNA, Cyclin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PC10, monoclonal
species reactivity
human
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
General description
Proliferating cell nuclear antigen (PCNA) is a heterotrimeric nuclear protein that functions as a clamp on replicating DNA. In cases of DNA damage and failure of the DNA repair pathways, PCNA mediates translesion DNA synthesis, a DNA damage tolerance (DDT) pathway, which allows replication to progress in spite of large lesions to DNA which may be caused by replication stress, ionizing radiation, spontaneous DNA damage, among other factors. PCNA primarily recruits low-fidelity DNA polymerase enzymes that are capable of elongating DNA over lesion sites. PCNA is regulated by cycles of ubiquitination, depending on the DDT pathway involved: PCNA is monoubiquitinated by Rad6–Rad18; polyubiquitinated by the Mms2-Ubc13-Rad5; or sumoylated by the Ubc9-Siz1 complex.
Immunogen
Recombinant protein corresponding to human PCNA.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCNA in human colon adenocarcinoma tissues and human tonsil tissues.
Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody) validated in WB, IHC, IF to detect PCNA also known as Proliferating cell nuclear antigen, PCNA, Cyclin.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody recognized PCNA in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody recognized PCNA in 10 µg of HeLa cell lysate.
Target description
~29 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rindert Missiaen et al.
Cell metabolism, 34(8), 1151-1167 (2022-07-16)
Hepatocellular carcinoma (HCC) is a typically fatal malignancy exhibiting genetic heterogeneity and limited therapy responses. We demonstrate here that HCCs consistently repress urea cycle gene expression and thereby become auxotrophic for exogenous arginine. Surprisingly, arginine import is uniquely dependent on
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Cell reports, 42(1), 112013-112013 (2023-01-20)
Clinical sequencing efforts are rapidly identifying sarcoma gene fusions that lack functional validation. An example is the fusion of transcriptional coactivators, VGLL2-NCOA2, found in infantile rhabdomyosarcoma. To delineate VGLL2-NCOA2 tumorigenic mechanisms and identify therapeutic vulnerabilities, we implement a cross-species comparative
Hai-Yan Wu et al.
NPJ Regenerative medicine, 6(1), 36-36 (2021-07-01)
Cardiovascular disease is the leading cause of death in the world due to losing regenerative capacity in the adult heart. Frogs possess remarkable capacities to regenerate multiple organs, including spinal cord, tail, and limb, but the response to heart injury
Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish.
Helen Eachus et al.
Communications biology, 7(1), 416-416 (2024-04-06)
Exposure to excess glucocorticoid (GC) during early development is implicated in adult dysfunctions. Reduced adult hippocampal neurogenesis is a well-known consequence of exposure to early life stress or elevated GC, however the effects on neurogenesis during development and effects on
Jonathan Ribeiro et al.
Nucleic acids research, 51(10), 4791-4813 (2023-03-16)
Recycling and de-novo deposition of histones during DNA replication is a critical challenge faced by eukaryotic cells and is coordinated by histone chaperones. Spermatogenesis is highly regulated sophisticated process necessitating not only histone modification but loading of testis specific histone
Articles
Loading controls in western blotting application.
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