NA02A

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

suspension (Liquid), clone K121, Calbiochem^®

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: K121, monoclonal
• Species reactivity: human, mouse, rat
• Application: —
• Citations: 9

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: K121, monoclonal
• form: suspension (Liquid)
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Affinity Purification ; Immunoprecipitation (15 µL beads/sample); Immunoprecipitation (1-2 µg/mL); Immunocytochemistry ; Paraffin Sections)
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).

Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

Immunogen

2,2,7-trimethylguanosine

Application

Affinity Purification (see application references)

Immunoprecipitation (15 µl beads/sample)

Immunoprecipitation (1-2 µg/ml; see application references)

Immunocytochemistry (use Cat. No. NA02)

Paraffin Sections (use Cat. No. NA02)

Physical form

In PBS.

Analysis Note

Negative Control
Transfer RNA (tRNA)

Positive Control
Heterogenous nuclear RNA (hnRNA)

Other Notes

Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5′ cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.

Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem.269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable