MABE302

Anti-2,2,7-Trimethylguanosine Antibody, clone K121

clone K121, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: K121, monoclonal
• Species reactivity: human, mouse
• Application: ICC, IP, affinity chromatography
• Citations: 13

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: K121, monoclonal
• species reactivity: human, mouse
• technique(s): affinity chromatography: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

The 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.

Application

Anti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.

Immunoaffinity Purification Analysis: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).

Physical form

Format: Purified

Analysis Note

Control
NIH/3T3, HeLa, and A431 cells

Evaluated by Immunocytochemistry in NIH/3T3, HeLa, and A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable