MABF33

Anti-MHC class II (I-A/I-E) Antibody, clone M5/114

clone M5/114, from rat

• Synonym(s): CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: M5/114, monoclonal
• Application: FACS, IHC, WB
• Citations: 12

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: M5/114, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001036070
• UniProt accession no.: P04441
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd74(16149)

Description

General description

MHC class II-associated invariant chain (I-A/I-E), also known as Ia antigen-associated invariant chain, are single-pass type II membrane glycoproteins containing a single thyroglobulin type-I domain. They are critical components in the processing of MHC class II antigen through forming a stabilizing complex following the alpha/beta heterodimer synthesis, and orchestrating complex trafficking away from the ER compartments in which class II peptide loading occurs.

~32 kDa observed.
There are 2 isoforms produced by alternative splicing: 32 kDa Isoform Long and 24 kDa Isoform Short.

Application

Detect MHC class II (I-A/I-E) using this Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC on spleen cells. (Bhattacharya, A., et al. (1981). The Journal of immunology. 127(6):2488-2496.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH on thymus tissue. (de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MHC class II (I-A/I-E) in 10 µg of mouse spleen tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable