MABF80C

Anti-MHC class II (I-A/I-E), APC, clone M5/114 .15.2 Antibody

clone M5/114.15.2, from rat, Allophycocyanin conjugate

• Synonym(s): H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain (Ii), MHC class II-associated invariant chain, CD74, MHC class II (I-A/I-E)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: Allophycocyanin conjugate
• Clone: M5/114.15.2, monoclonal
• Application: FACS
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: Allophycocyanin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: M5/114.15.2, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: P04441
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd74(16149)

Description

General description

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, APC, clone M5/114 .15.2, Cat. No. MABF806 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable