MABE941

Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody

clone 5D10D4, from rat

• Synonym(s): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 5D10D4, monoclonal
• Species reactivity: human
• Application: ChIP, DB, ELISA, ICC, WB
• Citations: 1

Properties

• biological source: rat
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5D10D4, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable (ChIP-seq), ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_003520
• UniProt accession no.: P68431
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer28)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.

~ 17 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human phospho Histone H3.1 (Ser28).

Application

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides.

Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013)
ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in a nocodozole treated HeLa cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: MABE13

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable