MABE952

Anti-Histone H3.1 Antibody

rat monoclonal, 1D4F2

• Synonym(s): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 1D4F2, monoclonal
• Species reactivity: human, mouse
• Application: ChIP, ICC, IP, WB
• Citations: -

Properties

• Product Name: Anti-Histone H3.1 Antibody, clone 1D4F2, clone 1D4F2, 1 mg/mL, from rat
• biological source: rat
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D4F2, monoclonal
• species reactivity: human, mouse
• concentration: 1 mg/mL
• technique(s): ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_003520
• UniProt accession no.: P68431
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~17 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.1.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Histone H3.1 in HeLa cells.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).

Alexa Fluor^™ is a registered trademark of Life Technologies.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

This Anti-Histone H3.1 Antibody, clone 1D4F2 is validated for use in western blotting, ICC, IP, ChIP & ChIP-seq for the detection of Histone H3.1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1