ABE559

Anti-Xrcc1 Antibody

serum, from rabbit

• Synonym(s): DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 1

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_033558
• UniProt accession no.: Q60596
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... XRCC1(7515)

Description

General description

DNA repair protein XRCC1 (X-ray repair cross-complementing protein 1) is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells.

~80 kDa observed. The molecular weight of this protein is calculated at 69 kDa; however it has been observed at ~80 kDa (Lee, Y., et al. (2009). Nature Neurosci. 12:(8):973-980.). An uncharacterized band may be observed at ~51 kDa in some cell lysates.

Immunogen

Linear peptide corresponding to mouse Xrcc1.

Application

Anti-Xrcc1 Antibody is a highly specific rabbit polyclonal antibody, that targets XRCC1 & has been tested in western blotting & IP.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Xrcc1 in HepG2 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected Xrcc1 in HEK293T, HeLa and MEF cell lysates (Lee, Y., et al. (2009). Nature Neurosci. 12:(8):973-980.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Xrcc1 in HEK293T, HeLa and MEF cell lysates (Lee, Y., et al. (2009). Nature Neurosci. 12:(8):973-980.).

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Xrcc1 in 10 µg of Jurkat cell lysate.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1