MABE2020

Anti-XRCC1 Antibody, clone 33-2-5

• Synonym(s): DNA repair protein XRCC1;X-ray repair cross-complementing protein 1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 33-2-5, monoclonal
• Application: IP, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 33-2-5, monoclonal
• mol wt: calculated mol wt 69.5 kDa, observed mol wt ~80 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• epitope sequence: Unknown
• Protein ID accession no.: NP_006288
• UniProt accession no.: P18887
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... XRCC1(18189)

Description

General description

DNA repair protein XRCC1 (UniProt: P18887; also known as X-ray repair cross-complementing protein 1) is encoded by the XRCC1 gene (Gene ID: 7515) in human. XRCC1 is a scaffold protein that is involved in DNA single-strand break repairs by mediating the assembly of DNA break repair protein complexes. Upon detection of DNA damage, it moves from the nucleoli to the global nuclear chromatin. It specifically interacts with nicked and gapped DNA and acts as a nick sensor capable of interacting with a variety of other proteins involved in repair, including DNA polymerases and DNA ligases. It contains three distinct domains: the N-terminal domain, and two independent BRCT domains (aa 315-403 and 539-629), connected by two intervening unstructured linkers that serve as critical interacting platforms for various repair factors. The first BRCT domain is reported to interact with PARP1, APE1, MPG, NTH1 and NEIL1 and the second BRCT domain interacts with DNA ligase III. It is shown to negatively regulate ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. (Ref.: Mok, MCY., et al. (2019). Sci. Rep. 9; Article 3095; Caldecott, KW., et al. (1995). Nucl, Acids Res. 23(3); 4836-4843).

Immunogen

His-tagged full-length recombinant human XRCC1 expressed in E.coli.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XRCC1 in HeLa cell nuclear extract.

Tested Applications

Western Blotting Analysis: A representative lot detected XRCC1 in Western Blotting application (Caldecott, K.W., et al. (1995). Nucleic Acids Res. 23(23):4836-43).

Immunoprecipitation Analysis: A representative lot immunoprecipitated XRCC1 in Immunoprecipitation application (Caldecott, K.W., et al. (1995). Nucleic Acids Res. 23(23):4836-43).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 33-2-5 is a mouse monoclonal antibody that detects XRCC1.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable