344905

FURA 2/AM

Cell-permeable ester form of FURA 2.

• Synonym(s): FURA 2/AM, 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester
• Empirical Formula (Hill Notation): C₄₄H₄₇N₃O₂₄
• CAS Number: 108964-32-5
• Molecular Weight: 1001.85
• UNSPSC Code: 12171500
• NACRES: NA.47
• MDL number: MFCD00036976

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• fluorescence: λ_ex 335 nm (high), λ_ex 362 nm (low), λ_em 505 nm (high), λ_em 512 nm (low)
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N(CC(=O)OCOC(=O)C)(CC(=O)OCOC(=O)C)c1c(cc(cc1)C)OCCOc2c3c([o]c(c3)c4ncc([o]4)C(=O)OCOC(=O)C)ccc2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• InChI key: UTBYGIDPBQOHNT-UHFFFAOYSA-N

Description

General description

Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.

Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.

PLURONIC^® F-127, PROTEIN GRADE Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Ratiometric calcium indicator

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.

Further dilute with aqueous buffers just prior to use.

Other Notes

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pluronic is a registered trademark of BASF

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable